说明书
MSDS产品描述：Rabbit polyclonal antibody to GPR176免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GPR176. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：GPR176相关名称：Probable G-protein coupled receptor 176; HB-954
基因编号(人)：
11245;
基因编号(小鼠)：
381413;
基因编号(大鼠)：
117257;
蛋白编号(人)：
Q14439;
蛋白编号(小鼠)：
Q80WT4;
蛋白编号(大鼠)：
Q64017;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GPR176 expression in HEK293T (A), Hela (B), U2OS (C), mouse testis (D), mouse heart (E), rat testis (F), rat heart (G) whole cell lysates. (Predicted band size: 56 kD; Observed band size: 57 kD) -
Immunofluorescent analysis of GPR176 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.