产品描述：Rabbit polyclonal antibody to NDC80免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NDC80. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：NDC80相关名称：HEC; HEC1; KNTC2; Kinetochore protein NDC80 homolog; Highly expressed in cancer protein; Kinetochore protein Hec1; HsHec1; Kinetochore-associated protein 2; Retinoblastoma-associated protein HEC 基因编号(人)： 10403; 基因编号(小鼠)： 67052; 蛋白编号(人)： O14777; 蛋白编号(小鼠)： Q9D0F1; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of NDC80 expression in mouse spleen (A), rat spleen (B) whole cell lysates. (Predicted band size: 73 kD; Observed band size: 74 kD)
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  Immunofluorescent analysis of NDC80 staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.