产品描述：Rabbit polyclonal antibody to MRCK alpha免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MRCK alpha. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：CDC42BPA相关名称：KIAA0451; Serine/threonine-protein kinase MRCK alpha; CDC42-binding protein kinase alpha; DMPK-like alpha; Myotonic dystrophy kinase-related CDC42-binding kinase alpha; MRCK alpha; Myotonic dystrophy protein kinase-like alpha 基因编号(人)： 8476; 基因编号(小鼠)： 226751; 基因编号(大鼠)： 114116; 蛋白编号(人)： Q5VT25; 蛋白编号(小鼠)： Q3UU96; 蛋白编号(大鼠)： O54874; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of MRCK alpha expression in C6 (A), BV2 (B), HEK293T (C), Panc1 (D), U87MG (E) whole cell lysates. (Predicted band size: 197 kD; Observed band size: 186 kD)
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  Immunofluorescent analysis of MRCK alpha staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).