产品描述：Rabbit polyclonal antibody to CLIP2免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CLIP2. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：CLIP2相关名称：CYLN2; KIAA0291; WBSCR3; WBSCR4; WSCR4; CAP-Gly domain-containing linker protein 2; Cytoplasmic linker protein 115; CLIP-115; Cytoplasmic linker protein 2; Williams-Beuren syndrome chromosomal region 3 protein; Williams-Beuren syndrome chromosomal region 4 protein 基因编号(人)： 7461; 蛋白编号(人)： Q9UDT6; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CLIP2 expression in AML12 (A), PC12 (B), A549 (C), LO2 (D), Hela (E) whole cell lysates. (Predicted band size: 115 kD; Observed band size: 130 kD)
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  Immunofluorescent analysis of Vimentin staining in CLIP2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).