产品描述：Rabbit polyclonal antibody to SOD1免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SOD1. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：SOD1相关名称：Superoxide dismutase [Cu-Zn]; Superoxide dismutase 1; hSod1 基因编号(人)： 6647; 基因编号(小鼠)： 20655; 基因编号(大鼠)： 24786; 蛋白编号(人)： P00441; 蛋白编号(小鼠)： P08228; 蛋白编号(大鼠)： P07632; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SOD1 expression in mouse liver (A), mouse testis (B), rat liver (C), rat testis (D) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 16 kD)
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  Immunofluorescent analysis of SOD1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).