产品描述：Rabbit polyclonal antibody to SUMO2/3免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SUMO2/3. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：SUMO2相关名称：SMT3A; SMT3H2; Small ubiquitin-related modifier 2; SUMO-2; HSMT3; SMT3 homolog 2; SUMO-3; Sentrin-2; Ubiquitin-like protein SMT3A; Smt3A 基因编号(人)： 6613; 基因编号(小鼠)： 170930; 基因编号(大鼠)： 690244; 蛋白编号(人)： P61956; 蛋白编号(小鼠)： P61957; 蛋白编号(大鼠)： P61959; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SUMO2/3 expression in HEK293T (A), A549 (B), U2OS (C), mouse brain (D), mouse testis (E), rat brain (F), rat testis (G) whole cell lysates. (Predicted band size: 10; 11 kD; Observed band size: 17 kD)
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  Immunofluorescent analysis of SUMO2/3 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).