产品描述：Rabbit polyclonal antibody to NF-kappaB p65 (Phospho-S536)免疫原：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S536 of human NF-kappaB p65 protein. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200), IP (1/10 - 1/100)基因名称：RELA相关名称：NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 基因编号(人)： 5970; 基因编号(小鼠)： 19697; 蛋白编号(人)： Q04206; 蛋白编号(小鼠)： Q04207; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of NF-kappaB p65 (Phospho-S536) expression in Hela (A), HEK293T (B), NIH3T3 (C), mouse kidney (D), H9C2 (E) whole cell lysates. (Predicted band size: 60 kD; Observed band size: 75 kD)
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  Immunohistochemical analysis of NF-kappaB p65 (Phospho-S536) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of NF-kappaB p65 (Phospho-S536) staining in rat lung. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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  Direct ELISA antibody dose-response curve using Anti-NF-kappaB p65 (Phospho-S536) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.