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CPA1420
  • Western blot analysis of FGFR1 (Phospho-Y654) expression in mouse testis (A) whole cell lysates. (Predicted band size: 91 kD; Observed band size: 120 kD)
  • Immunofluorescent analysis of FGFR1 (Phospho-Y654) staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
产品名称:Anti-FGFR1 (Phospho-Y654) Antibody
货号:CPA1420
来源:Rabbit
反应物种:H, M, R, C, P, Rb, Z
实验应用:WB, IF/IC
*反应物种注解:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*实验应用注解:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
规格
价格(元)
200 μl
4200
100 μl
2600
30 μl
1300
50 μl
1700
Ship in 3 days
产品描述:Rabbit polyclonal antibody to FGFR1 (Phospho-Y654)
免疫原:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y654 of human FGFR1 protein. The exact sequence is proprietary.
纯化方式:The antibody was purified by immunogen affinity chromatography.
克隆类型:Polyclonal
产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
稀释比:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)
基因名称:FGFR1
相关名称:BFGFR; CEK; FGFBR; FLG; FLT2; HBGFR; Fibroblast growth factor receptor 1; FGFR-1; Basic fibroblast growth factor receptor 1; BFGFR; bFGF-R-1; Fms-like tyrosine kinase 2; FLT-2; N-sam; Proto-oncogene c-Fgr; CD331
基因编号(人): 2260;
基因编号(小鼠): 14182;
基因编号(大鼠): 79114;
蛋白编号(人): P11362;
蛋白编号(小鼠): P16092;
蛋白编号(大鼠): Q04589;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of FGFR1 (Phospho-Y654) expression in mouse testis (A) whole cell lysates. (Predicted band size: 91 kD; Observed band size: 120 kD)
  • Immunofluorescent analysis of FGFR1 (Phospho-Y654) staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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