说明书
MSDS产品描述：Rabbit polyclonal antibody to E2F6免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human E2F6. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100), ChIP (1/100 - 1/500)基因名称：E2F6相关名称：Transcription factor E2F6; E2F-6
基因编号(人)：
1876;
基因编号(小鼠)：
50496;
蛋白编号(人)：
O75461;
蛋白编号(小鼠)：
O54917;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of E2F6 expression in PC12 (A), A2780 (B), HEK293T (C), HCT116 (D) whole cell lysates. (Predicted band size: 31 kD; Observed band size: 35 kD) -
Immunohistochemical analysis of E2F6 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
ChIP analysis of Mouse ES cell nuclear lysate, incubated for 16 hours at 4°C. Cross-linking (X-ChIP) using formaldehyde for 10 minutes. Positive control: Tuba3. Negative Control: Beta-Actin. Detection step: Real-time PCR.
Dnmt3b recruitment through E2F6 transcriptional repressor mediates germ-line gene silencing in murine somatic tissues