产品描述：Rabbit polyclonal antibody to Cytochrome P450 1A1/2免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cytochrome P450 1A1/2. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：CYP1A1; CYP1A2相关名称：CYP1A1; Cytochrome P450 1A1; CYPIA1; Cytochrome P450 form 6; Cytochrome P450-C; Cytochrome P450-P1; CYP1A2; Cytochrome P450 1A2; CYPIA2; Cytochrome P(3)450; Cytochrome P450 4; Cytochrome P450-P3 基因编号(人)： 1543; 1544; 基因编号(小鼠)： 13076; 13077; 基因编号(大鼠)： 24296; 24297; 蛋白编号(人)： P04798; P05177; 蛋白编号(小鼠)： P00184; P00186; 蛋白编号(大鼠)： P00185; P04799; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Cytochrome P450 1A1/2 expression in MCF7 (A), HeLa (B) whole cell lysates. (Predicted band size: 18; 20; 58 kD; Observed band size: 58 kD)
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  Immunofluorescent analysis of Cytochrome P450 1A1/2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).