说明书
MSDS产品描述：Rabbit polyclonal antibody to Cathepsin G免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Cathepsin G. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：CTSG相关名称：Cathepsin G; CG
基因编号(人)：
1511;
蛋白编号(人)：
P08311;
蛋白编号(大鼠)：
P17977;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Cathepsin G expression in A375 (A), A549 (B), Raw264.7 (C), H9C2 (D) whole cell lysates. (Predicted band size: 28 kD; Observed band size: 40 kD) -
Immunofluorescent analysis of Cathepsin G staining in BV2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).