说明书
MSDS产品描述：Rabbit polyclonal antibody to CK1 gamma 2免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CK1 gamma 2. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)基因名称：CSNK1G2相关名称：CK1G2; Casein kinase I isoform gamma-2; CKI-gamma 2
基因编号(人)：
1455;
基因编号(小鼠)：
103236;
基因编号(大鼠)：
65278;
蛋白编号(人)：
P78368;
蛋白编号(小鼠)：
Q8BVP5;
蛋白编号(大鼠)：
Q62762;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CK1 gamma 2 expression in HeLa (A), Raw264.7 (B), PC12 (C), rat heart (D) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 47 kD) -
Immunofluorescent analysis of CK1 gamma 2 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).