产品描述：Rabbit polyclonal antibody to BRS3免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BRS3. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：BRS3相关名称：Bombesin receptor subtype-3; BRS-3 基因编号(人)： 680; 基因编号(大鼠)： 260319; 蛋白编号(人)： P32247; 蛋白编号(大鼠)： Q8K418; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of BRS3 expression in A549 (A), H9C2 (B), PC3 (C), Panc1 (D) whole cell lysates. (Predicted band size: 44 kD; Observed band size: 50 kD)
• 
  Immunofluorescent analysis of BRS3 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.