产品描述:Rabbit polyclonal antibody to BRCA1免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BRCA1. The exact sequence is proprietary.纯化方式:The antibody was purified by immunogen affinity chromatography.克隆类型:Polyclonal产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比:WB (1/500 - 1/1000), IH (1/100 - 1/200)基因名称:BRCA1相关名称:RNF53; Breast cancer type 1 susceptibility protein; RING finger protein 53
基因编号(人):
672;
基因编号(小鼠):
12189;
基因编号(大鼠):
497672;
蛋白编号(人):
P38398;
蛋白编号(小鼠):
P48754;
蛋白编号(大鼠):
O54952;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of BRCA1 expression in HeLa (A), mouse liver (B), rat liver (C) whole cell lysates. (Predicted band size: 7; 78-85; 202-210 kD; Observed band size: 210 kD)
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Immunohistochemical analysis of BRCA1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.