产品描述：Rabbit polyclonal antibody to BNIP2免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human BNIP2. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), FC (1/100 - 1/200)基因名称：BNIP2相关名称：NIP2; BCL2/adenovirus E1B 19 kDa protein-interacting protein 2 基因编号(人)： 663; 基因编号(小鼠)： 12175; 蛋白编号(人)： Q12982; 蛋白编号(小鼠)： O54940; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of BNIP2 expression in mouse testis (A), rat testis (B) whole cell lysates. (Predicted band size: 36 kD; Observed band size: 36 kD)
• 
  Immunofluorescent analysis of BNIP2 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).