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CPA1056
  • Western blot analysis of APC (Phospho-S2054) expression in HeLa (A), Caco2 (B), SW480 (C), MCF7 (D), SP2/0 (E), PC12 (F) whole cell lysates. (Predicted band size: 311 kD; Observed band size: 310 kD)
  • Immunohistochemical analysis of APC (Phospho-S2054) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
产品名称:Anti-APC (Phospho-S2054) Antibody
货号:CPA1056
来源:Rabbit
反应物种:H, M, R, Mk, P
实验应用:WB, IH
*反应物种注解:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*实验应用注解:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
规格
价格(元)
200 μl
4200
100 μl
2600
30 μl
1300
50 μl
1700
Ship in 3 days
产品描述:Rabbit polyclonal antibody to APC (Phospho-S2054)
免疫原:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S2054 of human APC protein. The exact sequence is proprietary.
纯化方式:The antibody was purified by immunogen affinity chromatography.
克隆类型:Polyclonal
产品形式:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
稀释比:WB (1/500 - 1/1000), IH (1/50 - 1/100)
基因名称:APC
相关名称:DP2.5; Adenomatous polyposis coli protein; Protein APC; Deleted in polyposis 2.5
基因编号(人): 324;
基因编号(大鼠): 24205;
蛋白编号(人): P25054;
蛋白编号(小鼠): Q61315;
蛋白编号(大鼠): P70478;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of APC (Phospho-S2054) expression in HeLa (A), Caco2 (B), SW480 (C), MCF7 (D), SP2/0 (E), PC12 (F) whole cell lysates. (Predicted band size: 311 kD; Observed band size: 310 kD)
  • Immunohistochemical analysis of APC (Phospho-S2054) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Mammalian diaphanous-related formin 1 regulates GSK3ß-dependent microtubule dynamics required for T cell migratory polarization
Journal PLoS One
IF 3.7
Application
Reactivity Mouse
PMID 24260404
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