说明书
MSDS产品描述：Rabbit polyclonal antibody to ALDOA免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ALDOA. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：ALDOA相关名称：ALDA; Fructose-bisphosphate aldolase A; Lung cancer antigen NY-LU-1; Muscle-type aldolase
基因编号(人)：
226;
基因编号(小鼠)：
11674;
基因编号(大鼠)：
24189;
蛋白编号(人)：
P04075;
蛋白编号(小鼠)：
P05064;
蛋白编号(大鼠)：
P05065;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of ALDOA expression in Hela (A), HGC27 (B), mouse spleen (C), mouse lung (D) whole cell lysates. (Predicted band size: 39 kD; Observed band size: 39 kD) -
Immunofluorescent analysis of ALDOA staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).