产品描述：Rabbit polyclonal antibody to ACTN3免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ACTN3. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Polyclonal产品形式：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)基因名称：ACTN3相关名称：Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking protein 基因编号(人)： 89; 基因编号(小鼠)： 11474; 蛋白编号(人)： Q08043; 蛋白编号(小鼠)： O88990; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of ACTN3 expression in HeLa (A), mouse muscle (B), rat muscle (C) whole cell lysates. (Predicted band size: 103 kD; Observed band size: 100 kD)
• 
  Immunofluorescent analysis of ACTN3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).