产品描述：Recombinant rabbit monoclonal antibody to GALT免疫原：KLH-conjugated synthetic peptide encompassing a sequence within human GALT protein. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Monoclonal产品形式：Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)基因名称：GALT相关名称：Galactose-1-phosphate uridylyltransferase; Gal-1-P uridylyltransferase; UDP-glucose--hexose-1-phosphate uridylyltransferase 基因编号(人)： 2592; 基因编号(小鼠)： 14430; 基因编号(大鼠)： 298003; 蛋白编号(人)： P07902; 蛋白编号(小鼠)： Q03249; 蛋白编号(大鼠)： P43424; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of GALT expression in K562 (A), Hela (B), MCF7 (C), mouse liver (D), mouse kidney (E), rat liver (F) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 43 kD)
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  Immunohistochemical analysis of GALT staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of GALT staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).