产品描述：Recombinant rabbit monoclonal antibody to VEGFR1免疫原：KLH-conjugated synthetic peptide encompassing a sequence within human VEGFR1 protein. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Monoclonal产品形式：Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：FLT1相关名称：FLT; FRT; VEGFR1; Vascular endothelial growth factor receptor 1; VEGFR-1; Fms-like tyrosine kinase 1; FLT-1; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; FLT; Vascular permeability factor receptor 基因编号(人)： 2321; 基因编号(小鼠)： 14254; 基因编号(大鼠)： 54251; 蛋白编号(人)： P17948; 蛋白编号(小鼠)： P35969; 蛋白编号(大鼠)： P53767; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of VEGFR1 expression in HEK293T (A), Jurkat (B), K562 (C), mouse kidney (D), mouse muscle (E), rat muscle (F) whole cell lysates. (Predicted band size: 150 kD; Observed band size: 180 kD)
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  Immunofluorescent analysis of VEGFR1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).