产品描述：Recombinant rabbit monoclonal antibody to IBA1免疫原：KLH-conjugated synthetic peptide encompassing a sequence within human IBA1. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Monoclonal产品形式：Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：AIF1相关名称：G1; IBA1; Allograft inflammatory factor 1; AIF-1; Ionized calcium-binding adapter molecule 1; Protein G1 基因编号(人)： 199; 基因编号(小鼠)： 11629; 基因编号(大鼠)： 29427; 蛋白编号(人)： P55008; 蛋白编号(小鼠)： O70200; 蛋白编号(大鼠)： P55009; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of IBA1 expression in Myla2059 (A), THP1 (B), mouse brain (C), mouse kidney (D), rat brain (E) whole cell lysates. (Predicted band size: 16 kD; Observed band size: 17 kD)
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  Immunofluorescent analysis of IBA1 staining in RAW264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).