产品描述:Recombinant rabbit monoclonal antibody to Dia 1免疫原:KLH-conjugated synthetic peptide encompassing a sequence within human Dia 1. The exact sequence is proprietary.纯化方式:The antibody was purified by immunogen affinity chromatography.克隆类型:Monoclonal产品形式:Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.稀释比:WB (1/500 - 1/1000), IH (1/50 - 1/200)基因名称:DIAPH1相关名称:DIAP1; Protein diaphanous homolog 1; Diaphanous-related formin-1; DRF1
基因编号(人):
1729;
蛋白编号(人):
O60610;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of Dia 1 expression in Jurkat (A), Hela (B), MCF7 (C) whole cell lysates. (Predicted band size: 141 kD; Observed band size: 155 kD)
-
Immunohistochemical analysis of Dia 1 staining in human ovarian formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).