产品描述：Recombinant rabbit monoclonal antibody to PAX6免疫原：KLH-conjugated synthetic peptide encompassing a sequence within human PAX6. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Monoclonal产品形式：Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：PAX6相关名称：AN2; Paired box protein Pax-6; Aniridia type II protein; Oculorhombin 基因编号(人)： 5080; 基因编号(小鼠)： 18508; 基因编号(大鼠)： 25509; 蛋白编号(人)： P26367; 蛋白编号(小鼠)： P63015; 蛋白编号(大鼠)： P63016; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of PAX6 expression in Hela (A), Jurkat (B), K562 (C), mouse brain (D), rat brain (E), rat muscle (F) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 46; 48KD)
• 
  Immunofluorescent analysis of PAX6 staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).