说明书
MSDS产品描述：Recombinant rabbit monoclonal antibody to HMGB1免疫原：KLH-conjugated synthetic peptide encompassing a sequence within human HMGB1. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Monoclonal产品形式：Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IH (1/50 - 1/200)基因名称：HMGB1相关名称：HMG1; High mobility group protein B1; High mobility group protein 1; HMG-1
基因编号(人)：
3146;
基因编号(小鼠)：
100862258;
15289;
基因编号(大鼠)：
25459;
蛋白编号(人)：
P09429;
蛋白编号(小鼠)：
P63158;
蛋白编号(大鼠)：
P63159;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HMGB1 expression in HEK293T (A), THP1 (B), MCF1 (C), mouse liver (D), kidney (E), rat liver (F), rat kidney (G) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 25 kD) -
Immunohistochemical analysis of HMGB1 staining in human lymphoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.