产品描述：Recombinant rabbit monoclonal antibody to KDELR1免疫原：KLH-conjugated synthetic peptide encompassing a sequence within human KDELR1. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Monoclonal产品形式：Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：KDELR1相关名称：ERD2.1; ER lumen protein retaining receptor 1; KDEL endoplasmic reticulum protein retention receptor 1; KDEL receptor 1; Putative MAPK-activating protein PM23 基因编号(人)： 10945; 基因编号(小鼠)： 68137; 基因编号(大鼠)： 361577; 蛋白编号(人)： P24390; 蛋白编号(小鼠)： Q99JH8; 蛋白编号(大鼠)： Q569A6; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of KDELR1 expression in HEK293T (A), Jurkat (B), PC3 (C), mouse liver (D), mouse kidney (E), rat liver (F), rat kidney (G) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 78KD)
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  Immunofluorescent analysis of KDELR1 staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).