产品描述：Recombinant rabbit monoclonal antibody to LC3-I/II免疫原：KLH-conjugated synthetic peptide encompassing a sequence within human LC3-I/II. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Monoclonal产品形式：Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：MAP1LC3A相关名称：Microtubule-associated proteins 1A/1B light chain 3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B LC3 A; Microtubule-associated protein 1 light chain 3 alpha 基因编号(人)： 84557; 基因编号(小鼠)： 66734; 基因编号(大鼠)： 362245; 蛋白编号(人)： Q9H492; 蛋白编号(小鼠)： Q91VR7; 蛋白编号(大鼠)： Q6XVN8; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of LC3-I/II expression in HEK293T (A), Hela (B), THP1 (C), mouse brain (D), mouse liver (E), rat brain (F) whole cell lysates. (Predicted band size: 14 kD; Observed band size: 16 kD)
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  Immunofluorescent analysis of LC3-I/II staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).