产品描述：Recombinant rabbit monoclonal antibody to Histone H2A (Acetyl-K9)免疫原：KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K9 of human Histone H2A protein. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Monoclonal产品形式：Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：HIST1H2AB; HIST1H2AE相关名称：H2AFM; H2AFA; Histone H2A type 1-B/E; Histone H2A.2; Histone H2A/a; Histone H2A/m 基因编号(人)： 3012; 8335; 蛋白编号(人)： P04908; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Histone H2A (Acetyl-K9) expression in H1762 (A), LO2 (B) whole cell lysates. (Predicted band size: 14 kD; Observed band size: 14 kD)
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  Immunofluorescent analysis of Histone H2A (Acetyl-K9) staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).