产品描述：Recombinant rabbit monoclonal antibody to TRPV5免疫原：KLH-conjugated synthetic peptide encompassing a sequence within human TRPV5. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Monoclonal产品形式：Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：TRPV5相关名称：ECAC1; Transient receptor potential cation channel subfamily V member 5; TrpV5; Calcium transport protein 2; CaT2; Epithelial calcium channel 1; ECaC; ECaC1; Osm-9-like TRP channel 3; OTRPC3 基因编号(人)： 56302; 基因编号(小鼠)： 194352; 蛋白编号(人)： Q9NQA5; 蛋白编号(小鼠)： P69744; 储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of TRPV5 expression in HEK293T (A), EC9706 (B), LOVO (C), mouse lung (D), mouse kidney (E) whole cell lysates. (Predicted band size: 82 kD; Observed band size: 85-100 kD)
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  Immunofluorescent analysis of TRPV5 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).