产品描述:Recombinant rabbit monoclonal antibody to SHH免疫原:KLH-conjugated synthetic peptide encompassing a sequence within human SHH. The exact sequence is proprietary.纯化方式:The antibody was purified by immunogen affinity chromatography.克隆类型:Monoclonal产品形式:Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.稀释比:WB (1/500 - 1/1000), IH (1/50 - 1/200)基因名称:SHH相关名称:Sonic hedgehog protein; SHH; HHG-1
基因编号(人):
6469;
基因编号(小鼠):
20423;
蛋白编号(人):
Q15465;
蛋白编号(小鼠):
Q62226;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SHH expression in EC9706 (A), mouse liver (B) whole cell lysates. (Predicted band size: 49 kD; Observed band size: 51 kD)
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Immunohistochemical analysis of SHH staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).