说明书
MSDS产品描述：Recombinant rabbit monoclonal antibody to HAPLN1免疫原：KLH-conjugated synthetic peptide encompassing a sequence within human HAPLN1. The exact sequence is proprietary.纯化方式：The antibody was purified by immunogen affinity chromatography.克隆类型：Monoclonal产品形式：Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.稀释比：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)基因名称：HAPLN1相关名称：CRTL1; Hyaluronan and proteoglycan link protein 1; Cartilage-linking protein 1; Cartilage-link protein; Proteoglycan link protein
基因编号(人)：
1404;
基因编号(小鼠)：
12950;
基因编号(大鼠)：
29331;
蛋白编号(人)：
P10915;
蛋白编号(小鼠)：
Q9QUP5;
蛋白编号(大鼠)：
P03994;
储存效期：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HAPLN1 expression in EC9706 (A), mouse brain (B), rat brain (C) whole cell lysates. (Predicted band size: 40 kD; Observed band size: 48 kD) -
Immunofluorescent analysis of HAPLN1 staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).