产品描述:Recombinant rabbit monoclonal antibody to BCL2免疫原:Recombinant fusion protein of human BCL2. The exact sequence is proprietary.纯化方式:The antibody was purified by immunogen affinity chromatography.克隆类型:Monoclonal产品形式:Liquid in PBS, pH 7.3, 50% glycerol, and 0.05% Proclin300.稀释比:WB (1/500 - 1/1000), IH (1/100 - 1/500)基因名称:BCL2相关名称:Apoptosis regulator Bcl-2
基因编号(人):
596;
基因编号(小鼠):
12043;
蛋白编号(人):
P10415;
蛋白编号(小鼠):
P10417;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of BCL2 expression in HEK293T (A), Hela (B), Jurkat (C), mouse kidney (D) whole cell lysates. (Predicted band size: 26 kD; Observed band size: 26 kD)
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Immunohistochemical analysis of BCL2 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunohistochemical analysis of BCL2 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.