产品描述:Recombinant rabbit monoclonal antibody to Cytokeratin 7免疫原:Recombinant fusion protein of human Cytokeratin 7. The exact sequence is proprietary.纯化方式:The antibody was purified by immunogen affinity chromatography.克隆类型:Monoclonal产品形式:Liquid in PBS, pH 7.3, 50% glycerol, and 0.05% Proclin300.稀释比:WB (1/500 - 1/1000), IH (1/100 - 1/200)基因名称:KRT7相关名称:SCL; Keratin, type II cytoskeletal 7; Cytokeratin-7; CK-7; Keratin-7; K7; Sarcolectin; Type-II keratin Kb7
基因编号(人):
3855;
蛋白编号(人):
P08729;
储存效期:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Cytokeratin 7 expression in SGC7901 (A) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 54 kD)
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Immunohistochemical analysis of Cytokeratin 7 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Western blot analysis of Cytokeratin 7 expression in wild type (WT) and knockdown (KD) HeLa cell lysates.