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CIJ0002
产品名称:hsa-let-7b-5p miRNA Antagomir
货号:CIJ0002
来源:Synthetic
反应物种:H
实验应用:
*反应物种注解:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*实验应用注解:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
规格
价格(元)
10 nmol
3000
20 nmol
4500
Ship in 1 week
产品描述:Synthetic miRNA Antagomir is used to inhibit the activity of target hsa-let-7b-5p mRNA.
产品形式:Lyophilized powder
基因名称:hsa-let-7b-5p
基因编号(人): MIMAT0000063;
使用指导:Briefly centrifuge tubes containing miRNA antagomir to ensure that the miRNA pellet is located at the bottom of the tube. Dissolve miRNA antagomir to a convenient stock concentration using the recommended volume of DEPC H2O (or RNase-free water). For example: dissolve 10 nmol miRNA antagomir to 20 µM using 500 µl DEPC H2O (or RNase-free water). Pipette the solution up and down 3-5 times (or vortex briefly). Briefly centrifuge tubes containing miRNA antagomir to ensure that the solution is collected at the bottom of the tube. Aliquot the miRNA antagomir into small volumes and store at ≤ -20°C. miRNA antagomir is stable (for 6 months under the specified storage condition). For best results, use in 3 months and limit freeze-thaw events for each tube no more than five times.
组分:This synthetic miRNA is based on the mature miRNA sequence. The strand of the antagomir has 2 phosphorothioates at the 5' end, 4 phosphorothioates, 1 cholesterol group at the 3' end, and full-length nucleotide 2'-methoxy modification. Stability of miRNA antagomir appears to be significantly higher than miRNA inhibitors. It exhibits enhanced cellular uptake, stability and regulatory activity and is recommended for miRNA functional studies in vitro and in vivo.
储存效期:Shipped at 4 °C. Store at -20 °C for one year. Avoid freeze-thaw cycles after reconstitution.
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